MADRID - Valencia inflicted Barcelona's first league defeat at the Camp Nou since April 2012 as they came from behind to win 3-2 yesterday.
Alexis Sanchez had given the hosts a deserved early lead, but goals just before and after half-time from Dani Parejo and Pablo Piatti turned the game on its head.
Lionel Messi quickly levelled from the penalty spot, only for Paco Alcacer to strike the winner low past Victor Valdes five minutes later.
And Barca's day was summed up 13 minutes from time when Jordi Alba was sent-off for a second bookable offence.
Both Atletico Madrid and Real Madrid now have the chance to end Barca's record run of 59 league games at the top of the table when they face Real Sociedad and Athletic Bilbao respectively on Sunday.
Valencia came into the game off the back of a busy couple of days in the transfer market as five players left and four arrived in the final 48 hours of the January window.
None of Los Che's new men were signed in time to make the starting line-up, though, and they suffered a terrible start as Sanchez's shot looped up over the stranded Alves after just seven minutes.